Istvan Toth
The University of Queensland, Australia
Title: Polyelectrolyte coated liposome delivery systems against group A Streptococcus (GAS) infection
Biography
Biography: Istvan Toth
Abstract
We have developed an oral vaccine delivery system to prevent infection by GAS by encapsulating lipid core peptide (LCP) antigens into the liposomes. We synthesised the LCP construct by attaching C-16 lipoamino acid to J-14 (B-cell epitope derived from GAS M-protein) and P25 (CD4+ T helper cell epitope) using a microwave assisted solid phase peptide synthesis method. Liposomes were formulated incorporating the LCP and optimized for charge and lipid content using a thin film formation method. Optimized liposomes were coated with oppositely charged polyelectrolytes (positively charged trimethyl chitosan (TMC) and negatively charged sodium alginate) in a layer-by-layer approach. Optimized formulations were investigated for their efficiency of uptake by intestinal immune cells and ability to induce mucosal IgA and systemic IgG responses.
