Nitin Sharma
Meerut Institute of Engineering & Technology, India
Title: Enhancement in brain uptake of Vitamin D 3 nanoemulsion for treatment of cerebral ischemia:Formulation, Gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat model
Biography
Biography: Nitin Sharma
Abstract
In the present study, an attempt was made to develop vitamin-D3loaded nanoemulsion formulation for effective treatment of cerebral ischemia through nose to brain targeting. Tween 20 and polyethylene glycol (PEG-400) were chosen as surfactant/co-surfactant, while oleic acid as oil phase in nanoemulsion. The formulation was characterized for several in-vitro studies and further targeting efficiency of radiolabelled vitamin D3nanoemulsion (99mTc-Vitamin D-Nanoemulsion) was investigated through radiometry and gammascintigraphy. Efficacy of Vitamin D-Nanoemulsion was studied in transient middle cerebral artery occlusion rat model using Magnetic resonance images (MRI). Prepared Vitamin D-Nanoemulsion showed mean size range 49.29±10.28 nm and zeta potential 13.77. Gamma scintigraphy static images revealed brain deposition through nasal delivery of prepared formulation. Radiometry assay confirms approximate 4 fold increased biodistribution of 99mTc-Vitamin D-Nanoemulsion into brain compare to oil solution of 99mTc-Vitamin D. MRI of rat ischemic model confirmed better efficacy of Vitamin D-Nanoemulsion. Further elevated serum levelof MDA and decreased SOD, GSH and catalase activities confirm neurological deficits.