15^th International Conference and Exhibition on Nanomedicine and Pharmaceutical Nanotechnology March 18-19, 2019 HOLIDAY INN PARIS - PORTE DE CLICHY

Angie Andrea Bedoya Rodríguez has completed her MSc in Biochemistry and currently is a PhD student in Biotechnology at National University of Colombia. The current work belongs to her thesis topic.

Oncolytic virus therapy is a breakthrough in cancer treatment. Rotavirus is the leading cause of gastroenteritis and a large part of the population has immunological memory against it. Therefore, it is necessary to look for a biomaterial that permits shielding of WT1-5 oncolytic rotavirus in order to elude the antibody recognition and reach the tumor satisfactorily. For this, we used red blood cells (RBC), peripheral blood mononuclear cells (PBMC) or platelets as potential oncolytic rotavirus carriers. To encapsulate or bind virus to these cells, WT1-5 was incubated with cationic polyelectrolytes (polybrene, and protamine) or heparin and the formed nanocomplex (virus-polymer) was added to RBC, PBMC or platelets. RBC, PBMC or platelets loaded with WT1-5 were co-cultured with tumor cells (SK-MEL 28 or MCF-7) to evaluate the infection. Likewise, the release of rotavirus bound or encapsulated in RBC, PBMC or platelets at different times was evaluated, for this, the loaded cells were incubated every 30 min, centrifuged and the supernatant was recovered, which was added to the tumor cells. RBC loaded with WT1-5, by means of polybrene, increased the infection of tumor cells more than threefold with respect to positive control (WT1-5 incubated directly with tumor cells). With PBMC and platelets, similar results were obtained. We used another method, RBC-derived purified membranes or RAFTs-coated rotavirus WT1-5, which was added to tumor cells to evaluate infection. Both effectively coated rotavirus WT1-5, kept in suspension (carry), avoided antibody recognition, and allowed the infection of the tumor cells. Peripheral blood cells are promising cell carriers for efficient delivery of WT1-5 oncolytic rotavirus. Currently, we are also developing nanocomplexes between the rotavirus and alginate gel matrix system yielding good results.

Background: Silver colloids, silver nanoparticles suspended in liquid exposure properties Chemical, optical and antimicrobial materials widely used in a wide field of application. For these antibacterial and antifungal and antiviral qualities, psoriasis and eczema, these Specific properties are heavily influenced by particle size and shape. The methodology of the experiments plans is an optimal strategy allowing to organize the tests which accompany. A scientific research allows it to obtain the maximum of information with a maximum of precision from a minimum number of experiments in the fastest time and for the least cost[1] Our work consists in successfully synthesizing a colloidal solution of silver nanoparticles with the use of nitrate of silver as precursor and sodium citrate as reducing agent and optimizes the operating conditions. Materials & Methods: thermometer for solution, centrifuge, Analytical Balance, UV-spectrophotometer. [2] Observations: It should be noted that the process of formation and growth of nanoparticles can be easily affected by a multitude of factors, all the glassware must be cleaned by a solution of aqua regia. Results: The field of study allows to synthesize colloids whose mean particle size varies between 59.22nm and 111.85nm. Discussion: A change in the concentration of citrate in the colloid has an effect on the rate of reduction and on the nucleation / growth ratio. [3] Conclusion: synthesis of a silver colloid by chemical reduction using silver nitrate as a precursor and sodium citrate is easy to achieve.

Samia Bouledoua, Research Professor in Mineral Chemistry at the Faculty of Medicine, Université BADJI Mokhtar .Annaba (Algeria), is currently working on her PhD in Nanoscience and Nanotechnology

The nanoparticles have attracted great interest due to their unique physical and chemical properties, and zinc oxide (ZnO) is one of metal oxide nanoparticles, which attracts considerable attention due to its unique characteristics and its wide range of important technological applications. Nanocomposites are characterized by a combination of inorganic materials in the same particle. These assemblies have properties ranging from those of each of the constituents. The properties of ZnO nanoparticles can be improved depending on the interest of doping research with different metal atoms to meet specific needs and applications. Therefore, doping in the host matrix of zinc oxide nanoparticles with appropriate elements can adjust different properties of ZnO and is an effective method for improving and controlling its properties, which is crucial for its practical applications. Mg-doped ZnO is of considerable interest because of its unique optical, electrical and piezoelectric characteristics. Our work consists in the synthesis of a ZnO / Mg nanocomposite by a chemical coprecipitation method. The characterization of the synthesized nanocomposite was determined by X-ray diffraction (XRD) and UV-visible spectrophotometry. The DRX, on the one hand, makes it possible to determine the crystalline structure of the nanocomposite which is of wurtzite type, and secondly to calculate the size of crystallites obtained by the application of the Scherrer equation in the diameter of 49.44 nm. The UV visible spectrophotometry meanwhile revealed the existence of a maximum absorbance at 372 nm and allowed to assert that the synthesized powder corresponds to ZnO / Mg nanocomposites.

Alaimo A is a Young Assistant Researcher at the National Scientific and Technical Research Council (Spanish: Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET), the Argentine government agency, which directs and co-ordinates most of the scientific and technical research done in universities and institutes. She is a highly motivated and innovative Cellular and Molecular Biologist with specialization in Mitochondria biology, Molecular genetics, Cell biology, Biochemistry and Nanotechnology. She is an enthusiastic team player adept at providing leadership, while also learning from fellow team members.

Age-related macular degeneration (AMD) is a progressive retinal disease and is the leading cause of visual impairment in elderly people in western countries, for which there is still no cure. Genetics, environmental insults, and age-related issues are risk factors for the development of the disease and all these are linked to oxidative stress induction. Also, degeneration of retinal pigment epithelial cells (RPE) is a crucial causative factor responsible for the onset and progression of AMD. With the population aging, AMD will gradually become in a globally prevalent disease. The decline in the quality of life among affected patients highlights a critical need for developing preventive strategies. In this sense, phytochemicals with both antioxidant and anti-inflammatory properties may have a potential role in the prevention and treatment of this ocular disease. Particularly, there is an increased interest in the therapeutic effects of resveratrol (RSV). RSV is a naturally occurring polyphenolic compounds from the stilbenes family, mainly found in black grape and peanuts. RSV has beneficial effects on human health, including its neuroprotection, anti-inflammatory action, potent antioxidant activity and antiaging effects. Our research line is focused in investigating the protective effectiveness of RSV in an in vitro model of AMD. In addition, we designed and developed chitosan-based nanocarriers for RSV encapsulation to overcome physicochemical and pharmacokinetic limitations such as poor oral bioavailability, low water solubility and photosensibility. In summary, chitosan nanoparticles are a promising platform for ocular bioactives (eg. RSV) delivery for therapeutic intervention against oxidative stress-associated diseases, such as AMD

Oznur Aglar has completed her Master’s degree in Organic Chemistry at Umea University, Sweden, in 2016. She is currently a second-year PhD doctoral student in Analytical Chemistry at the University of Potsdam. Her current research focuses on the study of protein-ligand interactions, including binding affinity and binding kinetics by NMR.

Ceramide, an important component in the metabolism of sphingolipids, plays a significant role in proliferation, and apoptosis of cells. De novo synthesis of ceramide takes place at the cytosolic surface of the endoplasmic reticulum (ER), and then, ceramide is transferred to the Golgi apparatus for conversion into sphingomyelin and glucosphingolipids, mainly by nonvesicular trafficking. Non-vesicular transport of ceramide is carried out by the ceramide transfer protein (CERT) that consists of peptidic motifs and multiple domains. The C-terminal (StAR)- related lipid transfer (START) domain is the most important domain given the fact that it is capable of extracting and accommodating ceramide in its deep hydrophobic cavity. CERT could be an attractive pharmacological target because of its involvement in common pathological processes such as Alzheimer's disease, infectious diseases and cancer. A well-known antagonist of CERT is -N-(3-hydroxy-1-hydroxymethyl-3-phenylpropyl) dodecanamide (HPA-12), however, there is only limited structure-activity relationship (SAR) data available. In this study, we aim to explore the interaction between CERT and HPA-12 to establish SAR of this compound class by nuclear magnetic resonance spectroscopy (NMR) in order to improve the inhibition activity of the ligand for a potential drug design. Herein, we optimized the established expression and purification protocols to get a good yield of the monomer of the START domain. In order to get an idea about the suitability of the protein of interest for ligand and receptor-based NMR experiments, the target protein was labeled with 15N and purified using an optimized three step purification method. The 15N labeled START domain’s monomer was used to record initial 1H - 15N HSQC and TROSY NMR spectra in phosphate buffer. Results of both HSQC and TROSY experiments were promising for further receptor based NMR experiments