Angie Andrea Bedoya RodrÃguez
National University of Colombia, Colombia
Title: Development of nanocomplexes for the controlled delivery of WT1-5 virus with potential application in the treatment of cancer
Biography
Angie Andrea Bedoya RodrÃguez has completed her MSc in Biochemistry and currently is a PhD student in Biotechnology at National University of Colombia. The current work belongs to her thesis topic.
Abstract
Oncolytic virus therapy is a breakthrough in cancer treatment. Rotavirus is the leading cause of gastroenteritis and a large part of the population has immunological memory against it. Therefore, it is necessary to look for a biomaterial that permits shielding of WT1-5 oncolytic rotavirus in order to elude the antibody recognition and reach the tumor satisfactorily. For this, we used red blood cells (RBC), peripheral blood mononuclear cells (PBMC) or platelets as potential oncolytic rotavirus carriers. To encapsulate or bind virus to these cells, WT1-5 was incubated with cationic polyelectrolytes (polybrene, and protamine) or heparin and the formed nanocomplex (virus-polymer) was added to RBC, PBMC or platelets. RBC, PBMC or platelets loaded with WT1-5 were co-cultured with tumor cells (SK-MEL 28 or MCF-7) to evaluate the infection. Likewise, the release of rotavirus bound or encapsulated in RBC, PBMC or platelets at different times was evaluated, for this, the loaded cells were incubated every 30 min, centrifuged and the supernatant was recovered, which was added to the tumor cells. RBC loaded with WT1-5, by means of polybrene, increased the infection of tumor cells more than threefold with respect to positive control (WT1-5 incubated directly with tumor cells). With PBMC and platelets, similar results were obtained. We used another method, RBC-derived purified membranes or RAFTs-coated rotavirus WT1-5, which was added to tumor cells to evaluate infection. Both effectively coated rotavirus WT1-5, kept in suspension (carry), avoided antibody recognition, and allowed the infection of the tumor cells. Peripheral blood cells are promising cell carriers for efficient delivery of WT1-5 oncolytic rotavirus. Currently, we are also developing nanocomplexes between the rotavirus and alginate gel matrix system yielding good results.
Houmria Hamza
University of Badji Mokhtar, Algeria
Title: Silver Nanoparticles; Parametric study and modeling by application of the experimental design methodology
Biography
Abstract
Background: Silver colloids, silver nanoparticles suspended in liquid exposure properties Chemical, optical and antimicrobial materials widely used in a wide field of application. For these antibacterial and antifungal and antiviral qualities, psoriasis and eczema, these Specific properties are heavily influenced by particle size and shape. The methodology of the experiments plans is an optimal strategy allowing to organize the tests which accompany. A scientific research allows it to obtain the maximum of information with a maximum of precision from a minimum number of experiments in the fastest time and for the least cost[1] Our work consists in successfully synthesizing a colloidal solution of silver nanoparticles with the use of nitrate of silver as precursor and sodium citrate as reducing agent and optimizes the operating conditions. Materials & Methods: thermometer for solution, centrifuge, Analytical Balance, UV-spectrophotometer. [2] Observations: It should be noted that the process of formation and growth of nanoparticles can be easily affected by a multitude of factors, all the glassware must be cleaned by a solution of aqua regia. Results: The field of study allows to synthesize colloids whose mean particle size varies between 59.22nm and 111.85nm. Discussion: A change in the concentration of citrate in the colloid has an effect on the rate of reduction and on the nucleation / growth ratio. [3] Conclusion: synthesis of a silver colloid by chemical reduction using silver nitrate as a precursor and sodium citrate is easy to achieve.
