Congcong Lin
Hong Kong Baptist University, Hong Kong
Title: Targeted drug delivery with dual-ligand decorated liposomes by anti-CA IX antibody and tumor homing cell penetrating peptide in 2D and 3D lung cancer cells
Biography
Biography: Congcong Lin
Abstract
Statement of the Problem: Lung cancer is one of the most common lethal malignancies worldwide with 1.59 million deaths each year. Low accumulation of therapeutic agents in the tumor site and lack of penetration ability of a drug delivery system into tumor masses are the main obstacles in efficient lung cancer therapy.
Aim: The purpose of this study is to enhance the efficacy of anti-cancer drug against lung cancer by targeting carbonic anhydrase IX (CA IX) confined to cancerous cells to increase the accumulation at tumor sites and combining the advantage of tumor homing cell penetrating peptide (CPP33) to promote the penetration of the drug loaded liposomes into lung cancer cells.
Methodology & Theoretical Orientation: Anti-CA IX antibody and CPP33 dual-ligand triptolide-loaded liposomes (dl-TPL-lip) were developed using the post-insertion technique. The characteristics of dl-TPL-lip including particle size, entrapment efficiency and drug release property were carried out. The effects of dl-TPL-lip on NSCLC cells were evaluated by wound healing and apoptosis assay. Moreover, the penetrating ability and inhibition efficacy of dl-TPL-lip were further investigated using 3D tumor spheroids.
Findings: The dl-TPL-lip displayed the optimal efficacy in inducing apoptotic feature of NSCLC cells, which showed tunable size (137.6±0.8 nm), high encapsulation efficiency (86.3±2.6) and sustained release. The superior penetrating ability and inhibitor effect on 3D tumor spheroids were also observed for dl-TPL-lip.
Conclusion & Significance: The data suggest that dual-ligand modification with anti-CA IX antibody and CPP33 on the surface of liposomes endow great potential for lung cancer therapy.